Identification of Single Nucleotide Polymorphism Markers in the Laccase Gene of Shiitake Mushrooms (Lentinula edodes)

We identified single nucleotide polymorphism (SNP) markers in the laccase gene to establish a line-diagnostic system for shiitake mushrooms. A total of 89 fungal isolates representing four lines, including Korean registered, Korean wild type, Chinese, and Japanese lines, were analyzed. The results suggest that SNP markers in the laccase gene can be useful for line typing in shiitake mushrooms.

Arthroscopic Surgery of the Knee Through Ambulatory Care Unit Under General Anesthesia

PURPOSE: Though many patients who visit ambulatory care unit are performed arthroscopic surgery under local anesthesia, it is difficult to do it in some patients. By selecting the anesthetic agents, educating the patients preoperatively and controlling the pain carefully, we gained the good results in arthroscopic surgery of knee through ambulatory care unit under general anesthesia without complications. MATERIALS AND METHODS: From May 1998 to December 1999, arthroscapic surgery of the knee(menis-cectomy, synovectomy, debridement, loose body removal, bursa excision) was carried out under general anesthesia in 91 patients through ambulatory care unit. The anesthetic agents used in operation were propofol(52 cases) and ethrane(39 cases), 10cc of 0,25% bupivacaine with 1% lidocaine was injected in1o knee joint after arthroscopic surgery for pain control. RESULTS: The pain scores were improved from 12.5 points to 14.7 points at rest, from 9.5 points to 13 point at walking. Postoperatively, there were nausea(2 cases), chest pain(1 case), elevated blood pressure(2 case), redness(3 cases), pain(18 cases), effusion(27 cases). But these symptoms were improved without other complications. CONCLUSION: It is considered one of the comfortable, safe end reliable methods to perform arthroscopic surgery of knee through ambulatory care unit under general anesthesia.

Intraosseous Ganglion of Both Patellae: A Case Report

An intraosseous ganglion cyst, of which pathogenesis is unclear, is grossly and histologically identical to soft tissue ganglion. It predominates in the subchondral regions of tubular bones and is an osteolytic lesion that is well demarcated, radiographically. Pathologically, it is composed of a multilocular, thick, fibrous walled cyst-like structure filled with a slimy, mucin rich fluid. Curettage or curettage with bone graft is usually curative. Authors experienced a case diagnosed as intraosseous ganglia of both patellae. A 37-year-old man complained of both knee joint pains for about 4 month. About 1 year ago prior to this visit, he was treated one time because of the anterior knee pain. In the following X-rays, a well-defined osteolytic lesion involving the subchondral area of both patellae was showed. After CT and bone scan-ning, excision biopsy with curettage was performed. The thick, jelly-like mucinous fluid oozed out. His-tological findings were consistent with an intraosseous ganglion cyst. At 8 months follow-up, pain was completely resolved and other abnormal findings were not observed clinically.